Volume 4, Issue 1

Chemical composition and biological activity of essential oil of Chenopodium ambrosioides from Yemen

Author(s): Ali G Al-kaf, Rebecca A Crouch, Annika Denkert, Andrea Porzel, Othman SS Al-Hawshabi, Nasser A Awadh Ali*, William N Setzer, Ludger Wessjohann

Abstract: The chemical composition of the hydrodistilled leaf essential oil from Chenopodium ambrosioides L. growing wild in Yemen was determined by GC-MS analysis, and its cytotoxic, and general antioxidant potential were evaluated. Major compounds of C. ambrosioides oil were ascaridole (54.2%), isoascaridole (27.7%) and p-cymene (8.1%). At concentrations of 50 and 25 μg/mL, the essential oil showed cytotoxic activity against HT29 (human colon adenocarcinoma cells), with growth inhibition of 100 and 56% (± 3). The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 10.4 μg/mL. TLC-bioautographic assay was used to identify the acetylcholinesterase inhibitory effect, and ascaridole was isolated and characterized (ESIMS, 1H NMR, 13C NMR and HMBC) as the responsible constituent for anticholinesterase activity.

Download Full Article: Click Here