American Journal of Essential Oil and Natural Products


Volume 4, Issue 1

Chemical composition and biological activity of essential oil of Chenopodium ambrosioides from Yemen

Author(s): Ali G Al-kaf, Rebecca A Crouch, Annika Denkert, Andrea Porzel, Othman SS Al-Hawshabi, Nasser A Awadh Ali*, William N Setzer, Ludger Wessjohann

Abstract: The chemical composition of the hydrodistilled leaf essential oil from Chenopodium ambrosioides L. growing wild in Yemen was determined by GC-MS analysis, and its cytotoxic, and general antioxidant potential were evaluated. Major compounds of C. ambrosioides oil were ascaridole (54.2%), isoascaridole (27.7%) and p-cymene (8.1%). At concentrations of 50 and 25 μg/mL, the essential oil showed cytotoxic activity against HT29 (human colon adenocarcinoma cells), with growth inhibition of 100 and 56% (± 3). The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 10.4 μg/mL. TLC-bioautographic assay was used to identify the acetylcholinesterase inhibitory effect, and ascaridole was isolated and characterized (ESIMS, 1H NMR, 13C NMR and HMBC) as the responsible constituent for anticholinesterase activity.

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