American Journal of Essential Oils and Natural Products
2021, Vol. 9 Issue 4, Part A
Formulation of antiseptics based on essential oils from: Cananga odorata, Cinnamomum zeylanicum, Citrus sinensis, Eugenia caryophyllata and Mentha piperita
AUTHOR(S): Kameni Georgette Njila, Emilia Lyonga Mbamyah, Moni Ndedi Esther Del Florence, George Mondinde Ikomey, Ngongang Tchami Dimitri, Martha Tongo Mesembe and Nyegue Maximilienne Ascension
ABSTRACT:The Covid-19 pandemic is a stark reminder that the easiest way to stop the spread of hand germs is by washing the hands. The unavailability of hand washing facilities has led to the alternative use of hand antiseptics. The present study was carried out to formulate hand antiseptics with antimicrobial and fragrance properties based on combinations of essential oils (EOs). EOs were obtained by steam distillation of five plants using the Clevenger apparatus. These EOs were combined C1, C2 and C3 according to the Kaloustian method. The bioactivity of EOs and their combinations was tested by Agar disc diffusion and micro dilution methods against reference strains of bacteria and fungi. Hand sanitizers containing combinations of EOs as antimicrobials and fragrance agents were formulated following the guideline recommended by the World Health Organization in 2019 with slight modifications and sensory analysis evaluated by a panel of 50 trained individuals. The EOs obtained were 1.1 % for Eugenia caryophyllata, 0.55 % for Citrus sinensis, 0.33 % for Cinnamomum zeylanicum, 0.26 % for Cananga odorata and 0.052 % for Mentha piperita. The inhibition effect of EOs on solid medium was exhibited with inhibition diameters ranging from 0 ± 0 mm for Citrus sinensis against Escherichia coli to 15 ± 1.5 mm for Eugenia caryophyllata against Staphylococcus aureus. The highest MIC was Mentha piperita 25 mg/mL against Candida albicans meanwhile the lowest MIC was 0.390 mg/mL for Cinnamomum zeylanicum and Eugenia caryophyllata against all tested bacteria. The most active combination was C1 with MIC of 0.390 mg/mL on all six strains. The EO combinations were 16 times more active than the hand sanitizer tested. EOs extracted gave good antimicrobial and antifungal activities. Their fragrance was acceptable and thus can be used in hand sanitizers. This study has shown that the addition of EOs to the standard antiseptics potentiates its action against microbes which may have developed resistance to the standard formula.
Pages: 19-27 | 131 Views 18 Downloads
How to cite this article:
Kameni Georgette Njila, Emilia Lyonga Mbamyah, Moni Ndedi Esther Del Florence, George Mondinde Ikomey, Ngongang Tchami Dimitri, Martha Tongo Mesembe, Nyegue Maximilienne Ascension. Formulation of antiseptics based on essential oils from: Cananga odorata, Cinnamomum zeylanicum, Citrus sinensis, Eugenia caryophyllata and Mentha piperita. Am J Essent Oil Nat Prod 2021;9(4):19-27.