2021, Vol. 9 Issue 2, Part A
Antimicrobial, cytotoxic and mutagenic activities of Bauhinia holophylla hydroalcoholic extract
AUTHOR(S): Gabriel Davi Marena, Luiz Leonardo Saldanha, Matheus Aparecido dos Santos Ramos, Rone Aparecido De Grandis, Anne Lígia Dokkedal, Tais Maria Bauab, Fernando Rogério Pavan and Flávia Aparecida Resende
ABSTRACT:The hydroethanolic leaves extract of Bauhinia holophylla, from Fabaceae family, a Brazilian medicinal plant, have been studied as an alternative therapy for the diabetes treatment. In line with the effort to discover novel therapeutic applications, the present study aimed to investigate the antimicrobial activity of B. holophylla against microorganisms of clinical relevance. Also, we have sought to determine the cyto-genotoxicity potential to evaluate if the extract exhibit beneficial or harmful effect. The antimicrobial activity of B. holophylla was measured by the determination of the minimum inhibitory concentration (MIC; microdilution technique), and the cytotoxicity index (IC50) against the GM 07492, HeLa and HepG2 cell lines was used to calculate the selectivity index (SI). The mutagenicity was determined by the Ames test on Salmonella Typhimurium strains TA98, TA97a, TA100 and TA102. B. holophylla displayed weak activity against Staphylococcus aureus and Escherichia coli (MIC = 500 and 2000 μg mL-1, respectively). On the other hand, this extract was active against fungi of the Candida species. B. holophylla showed MIC values 15.6 μg mL-1 against C. glabrata, 62.5 μg mL-1 against C. krusei and C. tropicalis, and 125 μg mL-1 against C. parapsilosis and C. albicans. At these concentrations, the extract showed an absence of cytotoxicity in the human cells. However, the extract induces, at a much higher rate, frame shift mutations (TA98) in the Ames test. These results contribute to valuable data on the antimicrobial potential of B. hoplophylla but suggest caution in their use.
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How to cite this article:
Gabriel Davi Marena, Luiz Leonardo Saldanha, Matheus Aparecido dos Santos Ramos, Rone Aparecido De Grandis, Anne Lígia Dokkedal, Tais Maria Bauab, Fernando Rogério Pavan, Flávia Aparecida Resende. Antimicrobial, cytotoxic and mutagenic activities of Bauhinia holophylla hydroalcoholic extract. Am J Essent Oil Nat Prod 2021;9(2):01-06.