2018, Vol. 6 Issue 4, Part A
Evaluation of antidiabetic activity, metabolic profiling and determination of major metabolites by LC-ESI/MS/MS of Novobet
AUTHOR(S): Mizhgona Sharofova, Sodik Numonov, William N Setzer, Parviz Sukhrobov, Farukh Sharopov, Yusuf Nuraliev, Lan Jiang, Gafforzoda Latofat and Maidina Habasi
ABSTRACT:Diabetes mellitus is a heterogeneous disease developing under the result of the congenital combination and acquired factors, unhealthy lifestyles, especially wrong eating habits, hypokinesis and characterized by chronic progressive course leading to severe micro and macrovascular complications. Geranium collinum, Glycyrrhizaglabra, and Rhuscoriaria, are species growing in Tajikistan, and are characterized as natural sources of phenolic compounds that have several known biological activities and serve as a traditional medicine against various disorders including type 2 diabetic diseases. The chemical profile of "Novobet" was analyzed by LC-ESI-MS/MS. "Novobet" was screened for in vitro antidiabetic (PTP-1B enzyme inhibition) and antioxidant effects (DPPH radical scavenging). The in vivo antidiabetic and acute toxicity of "Novobet" was evaluated in animal models. Based on the pH of the medium, the new anti-diabetic phytomedicine, namely "Novobet", was developed. Glucose and lipids levels and the concentration of malondialdehyde (MDA) in serum of laboratory animals treated with "Novobet" were investigated. The blood sugar level in experimental rabbits, after treatment with 5mL/kg of "Novobet", was decreased after 7, 15 and 30 days, respectively, which indicated active hypoglycemic action. In addition, the level of total lipids in the blood decreased to 44.5%, and the level of blood cholesterol under the influence of the "Novobet" decreased to 32.2%. The MDA level of rabbit’s blood, after treatment with "Novobet" at the dose of 5mL/kg, decreased significantly. A total of 25 major compounds were identified in “Novobet” by LC-ESI-MS/MS spectroscopy. The new Phytomedicine "Novobet", exhibited potent antidiabetic and antioxidant activity and therefore it can serve as a natural antidiabetic and antioxidant nutraceutical.
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How to cite this article:
Mizhgona Sharofova, Sodik Numonov, William N Setzer, Parviz Sukhrobov, Farukh Sharopov, Yusuf Nuraliev, Lan Jiang, Gafforzoda Latofat, Maidina Habasi. Evaluation of antidiabetic activity, metabolic profiling and determination of major metabolites by LC-ESI/MS/MS of Novobet. Am J Essent Oil Nat Prod 2018;6(4):27-35.