American Journal of Essential Oils and Natural Products
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ISSN: 2321-9114, ICV 2016: 79.57

American Journal of Essential Oils and Natural Products

2016, Vol. 4 Issue 2, Part A

Antibacterial activity of essential oil of Trachyspermum ammi (L.) Sprague ex Turrill against isolated and standard bacteria

AUTHOR(S): S Omidpanah, M Vazirian, F Hosseinkhani, A Hadjiakhondi, M Pirali Hamedani, A Manayi
ABSTRACT:The essential oil of Trachyspermum ammi (L.) Sprague ex Turrill (Apiaceae) fruits was extracted by hydrodistillation. The residue of the fruit treated with hydrochloric acid and again hydrodistilled to extract volatile compounds. The components of obtained oils were identified using GC and GC-MS. Antibacterial activity of the oils and pure thymol were examined against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Enterococcus faecalis as well as two isolated bacteria, Salmonella typhimurium and E. coli, from diseased hen, using disk diffusion and microdilution methods. The fruit of the plant yielded 3.5 and 2.4 mL oils, with thirteen and twenty-four identified compounds in the oil before and after acid hydrolysis, respectively. Thymol, p-cymene, and γ-terpinene were the major components of the both oils. All the tested bacteria sufficiently suppressed by the oils with inhibition zone (IZD) diameters ranging 26.6-50.3 mm with minimum inhibitory concentration (MIC) of <0.02 μL/mL. Thymol inhibited growth of the bacteria with concentration of 100 mg/mL. The results of present study revealed that the fruit of T. ammi could serve for production of supplements or therapeutic agents for poultry industries.
Pages: 05-11  |  1244 Views  45 Downloads
How to cite this article:
S Omidpanah, M Vazirian, F Hosseinkhani, A Hadjiakhondi, M Pirali Hamedani, A Manayi. Antibacterial activity of essential oil of Trachyspermum ammi (L.) Sprague ex Turrill against isolated and standard bacteria. Am J Essent Oil Nat Prod 2016;4(2):05-11.
American Journal of Essential Oils and Natural Products