2014, Vol. 1 Issue 3, Part A
Composition and biological activities of the essential oil of Nigella sativa seeds isolated by accelerated microwave steam distillation with cryogenic grinding
AUTHOR(S): Rym Akloul, Farid Benkaci-Ali, Meriem Zerrouki, Gauthier Eppe
ABSTRACT:In this study, essential oil of Sahara Nigella sativa L. was extracted using a rapid extraction, the microwave steam distillation (MSD) and the cryogenic grinding (CG). Two procedures have been investigated, the MSD1 (seeds inside of oven apparatus) and MSD2 (seeds outside of oven apparatus). Forty-six compounds were identified and significant differences in quantities of the major constituents were observed, mainly were thymoquinone (CLG: 331.82-443.55 mg and CG: 272.95-413.57 mg/100 g of seeds), p-cymene (CLG: 181.71-244.17 mg, CG: 369.80- 374.40 mg/100 g of seeds), dehydro-sabina ketone (CLG: 24.60- 25.83 mg, GC: 44.02-50.69 mg/100g of seeds), carvacrol (CLG: 10.32-10.96 mg, CG: 3.91-12.67 mg/100 g of seeds) and longifolene (CLG: 11.90-16.43 mg, CG: 12.72-19.58 mg/100 g of seeds).Results showed that essential oils exhibit a good activity in each antioxidant system with a special attention for β-carotene bleaching test (IC50: 21 to 27 μg/ml) and reducing power (EC50: 9 to 14 μg/ml).The N. Sativa essential oils exhibited higher antibacterial and antifungal activities varying according to technique extraction and grinding mode used, with a high effectiveness against Gram-positive bacteria with a diameter of inhibition zones growth ranging from 9.5 to 35 mm and MIC and MBC values ranging from (0.042–0.10 mg/ml) to (0.20–0.75 mg/ml), respectively.
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How to cite this article:
Rym Akloul, Farid Benkaci-Ali, Meriem Zerrouki, Gauthier Eppe. Composition and biological activities of the essential oil of Nigella sativa seeds isolated by accelerated microwave steam distillation with cryogenic grinding. American Journal of Essential Oils and Natural Products. 2014; 1(3): 23-33.